Sanger Sequencing Service
The Gandel Charitable Trust Sequencing Centre uses Applied Biosystems 3130xl Genetic Analysers to generate read lengths of approximately 1,000 bases. pGEM and LongRead controls are included on every run to monitor sample processing and instrument performance, respectively.
- Sequencing and Electrophoresis (Submit template and primer together)
- Purification and Electrophoresis (Submit post-cycle sequencing reactions)
- Capillary Separation (Submit samples for electrophoretic separation only)
- All samples are submitted using our online service link ARIN
- Follow the sample submission instructions at the link above
- Samples can be delivered in person to the laboratory, sent by courier or mailed
- Please use padded envelopes when posting samples to prevent sample damage
- Samples received by 11 am each day will be processed in 24 hours
- Label tubes with sample name (<10 characters) and client name
DNA and primer requirements
Up to 1,000 bases can be sequenced per run; however, accurate read length is largely dependent upon several important factors. More Info
Data retrieval, evaluation and troubleshooting
Sequence data is available from the FTP site within 24 to 48 hours of sample receipt. More Info
To gain access to your data, click onto the Client FTP login and type in your Institute's username and password (available from the Facility).
Files are retained on the FTP site for a period of three months and all data is backed up to permanent archive.
It is essential to check base calling accuracy using the electropherogram as reference.
Manual editing may be required especially at the end of the run.
Service charges are sent out through monthly invoices.