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Whole transcriptome

For RNAseq gene expression studies investigating differential expression, we recommend a minimum of 30 million reads per sample using 50-100 bp fragment reads.

TruSeq® Stranded mRNA LT - poly-A enrichment

For RNAseq requiring the investigation of coding, non-coding regulation and alternative splice variants, 50-60 million reads per sample is recommended using 100 bp paired-end reads.

TruSeq® Stranded Total RNA LT -­ (with Ribo-­‐Zero Human/Mouse/Rat) - rRNA depletion

Ovation® RNA-Seq System V2 - SPIA technology

AmpliSeq Transcriptome

Total RNA that has been DNase treated (on column) needs to be supplied with a RIN score > 8 as determined by Agilent Bioanalyser analysis.